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Products:Tags & Cell Markers >> Fusion / Marker Proteins >> Alkaline Phosphatase
Overview
Product name
Anti-bacterial Alkaline Phosphatase antibody (HRP)
See all bacterial Alkaline Phosphatase products (3) ...
Description
Rabbit polyclonal to bacterial Alkaline Phosphatase (HRP)
Conjugation
HRP
Conjugation notes
Horseradish Peroxidase (HRP)
Tested applications
Dot Blot, ELISA, IHC-Fr, Immunomicroscopy, WBmore details
Cross reactivity
No reactivity ocurs against mammalian forms of Alkaline Phosphatase.
Immunogen
Full length protein (E. coli).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Fusion / Marker Proteins >> Alkaline Phosphatase
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Western blot - bacterial Alkaline Phosphatase antibody (HRP) (ab7319)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot Blot
IHC-Fr
Immunomicroscopy
Application notes
Immunoblotting (western or dot blot)
ELISA (1:3,500 to 1:15,000)
Immunoperoxidase electron microscopy
Immunohistochemistry
as well as other peroxidase-antibody based enzymatic assays.
Target
Relevance
Bacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.
Cellular localization
Periplasm
Alternative names
- Alkaline phosphatase antibody
- APase antibody
- BAP antibody
- PhoA antibody
Anti-bacterial Alkaline Phosphatase antibody (HRP) images:
Western blot - bacterial Alkaline Phosphatase antibody (HRP) (ab7319)
Anti-bacterial Alkaline Phosphatase antibody (HRP) (ab7319) at 1/3500 dilution + Purified protein from Mach1 Escherichia coli at 0.01 µg
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Exposure time : 15 seconds
This image is courtesy of an anonymous Abreview
References for Anti-bacterial Alkaline Phosphatase antibody (HRP) (ab7319)
This product has been referenced in:
- Szvetnik Aet al. Membrane topology of the GltS Na+/glutamate permease of Escherichia coli. FEMS Microbiol Lett 275:71-9 (2007).Read more (PubMed: 17662058) »
See 1 publication for this product
Publishing research using ab7319? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"