Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesImmunoblotting (western or dot blot)
ELISA (1:4,000 to 1:20,000)
as well as other peroxidase-antibody based enzymatic assays.
RelevanceBacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.
Alkaline phosphatase antibody
References for Anti-bacterial Alkaline Phosphatase antibody (Biotin) (ab7321)
has not yet been referenced specifically in any publications.
Publishing research using ab7321? Please let us know so that we can cite the reference in this datasheet.
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