Anti-bacterial Alkaline Phosphatase antibody (Biotin) (ab7321)

Overview

  • Product nameAnti-bacterial Alkaline Phosphatase antibody (Biotin)
  • Description
    Rabbit polyclonal to bacterial Alkaline Phosphatase (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISAmore details
  • Species reactivity
    No reactivity occurs against mammalian forms of Alkaline Phosphatase.
  • Immunogen

    Full length protein (E. coli).

  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Immunomicroscopy
WB
Dot blot
ELISA
  • Application notesImmunoblotting (western or dot blot)
    ELISA (1:4,000 to 1:20,000)
    Immuno microscopy
    Immunohistochemistry
    as well as other peroxidase-antibody based enzymatic assays.
  • Target

    • RelevanceBacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.
    • Cellular localizationPeriplasm
    • Alternative names
      • Alkaline phosphatase antibody
      • APase antibody
      • BAP antibody
      • PhoA antibody

    References for Anti-bacterial Alkaline Phosphatase antibody (Biotin) (ab7321)

    ab7321 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"