Anti-bacterial Luciferase antibody (HRP) (ab390)

Overview

  • Product name
    Anti-bacterial Luciferase antibody (HRP)
  • Description
    Rabbit polyclonal to bacterial Luciferase (HRP)
  • Conjugation
    HRP
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Luciferase from Photobacterium fischerii.
  • Tested applications
    Suitable for: WB, ELISA, Immunomicroscopymore details
  • Species reactivity
    Reacts with: Photobacterium
  • Immunogen

    Luciferase from Photobacterium fischerii.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against 0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.

References

This product has been referenced in:
  • Fransson M  et al. CAR/FoxP3-engineered T regulatory cells target the CNS and suppress EAE upon intranasal delivery. J Neuroinflammation 9:112 (2012). Read more (PubMed: 22647574) »

See 1 Publication for this product

Customer reviews and Q&As

Muchas gracias por enviarnos el cuestionario relleno y las imágenes.

Lamento muchísimo que el anticuerpo no os haya dado buenos resultados, y después de haber estudiado el protocolo enviado me gustaría haceros algu...

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Gracias por tu respuesta.

Es difícil evaluar la procedencia de las bandas sin más información.

Te agradecería que me mandaras los pesos moleculares en la imagen, así como información acerca ...

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Thank you for contacting us.

This antibody ab390 is produced against whole protein. It is not produced against a single epitope.

I am very sorry I couldn’t help you further. Please do not hesitate to contact us if you need any...

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Gracias por contactarnos y facilitarnos los detalles y las imágenes.

Lamento mucho que estéis teniendo problemas con este anticuerpo. Atendiendo a las imágenes enviadas, y siendo que el vial de anticuerpo se envió ...

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Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. Ab390 cross-reacts with Photobacterium fischerii.

Dear Shankar I am afraid that we don't have any references for the use of this antibody in immunohistochemistry. Perhaps if you give us some more details on the problems then we might be able to help.

Luciferase from photobacterium fischerii gives a 40 kDa band, but the band size of bacterial luciferases from other species are unknown to us.

This antibody reacts with bacterial luciferase (Photobacterium fischerii)showing an 80kDa band under non-reducing conditions and a 40kDa band under reducing conditions of SDS-PAGE.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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