|
|
|
|
Western blot - Bad antibody (ab13998)
(enlarge)
|
|
|
|
|
Product Name
|
Bad antibody
|
| |
See all Bad antibodies (29)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Chicken polyclonal to Bad
|
|
Immunogen
|
Recombinant fragment, corresponding to amino acids 2-168 of Human Bad.
|
|
Reacts with
(species key)
|
Hu, Rat
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: 1/500. Predicted molecular weight: 18 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Mitochondrion outer membrane. Cytoplasm. Note: Upon phosphorylation, locates to the cytoplasm.
|
|
Research areas
|
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
|
|
Relevance
|
Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Chicken
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgY
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: None
Constituents: PBS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bad antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Bad antibody images, references, products related to ab13998 and other tools.
|
| |
Bad antibody images:
|
 Western blot - Bad antibody (ab13998)
Bad antibody (ab13998) at 1/2000 dilution
Secondary
Goat anti-IgY-HRP at 1/1000 dilution
Predicted band size : 18 kDa
Please note that an E coli-derived fusion protein was used as the test antigen. Colorimetric method for signal development.
|
| |
| |
|
Search PubMed (MEDLINE) for references to Bad
|
| |
Bad antibody - more information
|
Customer reviews (feedback) regarding Bad antibody |
Customer FAQs regarding Bad antibody |
Protocols for Bad antibody |
Price and availability of products related to Bad antibody |
| |
Bad antibody - related products:
|
|
|
| |
Bad antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Bad antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
