• Product nameAnti-Bad antibody
    See all Bad primary antibodies
  • Description
    Rabbit polyclonal to Bad
  • SpecificityAb28840 recognises BAD.
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from human BAD around the phosphorylation site of Serine 75.

  • Positive control
    • IHC-P: breast carcinoma WB: extracts from 293 cells treated with forsklin.


Associated products


Our Abpromise guarantee covers the use of ab28840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 18 kDa).
ELISA 1/10000.


  • FunctionPromotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
  • Tissue specificityExpressed in a wide variety of tissues.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Post-translational
    Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
  • Cellular localizationMitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAD antibody
    • BAD_HUMAN antibody
    • BBC 2 antibody
    • BBC2 antibody
    • BBC6 antibody
    • Bcl 2 Antagonist of Cell Death antibody
    • Bcl 2 Binding Component 6 antibody
    • BCL X / BCL 2 Binding Protein antibody
    • BCL X Binding Protein antibody
    • Bcl XL/Bcl 2 Associated Death Promoter antibody
    • Bcl-2-binding component 6 antibody
    • Bcl-2-like protein 8 antibody
    • Bcl-XL/Bcl-2-associated death promoter antibody
    • Bcl2 antagonist of cell death antibody
    • BCL2 antagonist of cell death protein antibody
    • BCL2 associated agonist of cell death antibody
    • Bcl2 Associated Death Promoter antibody
    • BCL2 binding component 6 antibody
    • BCL2 binding protein antibody
    • Bcl2 Like 8 Protein antibody
    • Bcl2-L-8 antibody
    • BCL2L8 antibody
    • Proapoptotic BH3 Only Protein antibody
    see all

Anti-Bad antibody images

  • ab28840, at a dilution of 1/50, staining BAD in paraffin embedded breast carcinoma tissue by Immunohistochemistry.
    Left: untreated.
    Right: treated with synthesized peptide.
  • Anti-Bad antibody (ab28840) at 1/500 dilution + extracts from 293 cells (5-30ug) treated with forsklin

    Predicted band size : 18 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)

References for Anti-Bad antibody (ab28840)

This product has been referenced in:
  • Fiedler J  et al. MicroRNA-24 regulates vascularity after myocardial infarction. Circulation 124:720-30 (2011). WB, IHC-Fr ; Mouse . Read more (PubMed: 21788589) »

See 1 Publication for this product

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