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Synthetic peptide derived from the sequence of Mouse Bad, conjugated to KLH.
Our Abpromise guarantee covers the use of ab90435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa.|
|IHC-P||1/50. Antigen retrieval is not essential but may optimise staining.|
|ICC/IF||Use a concentration of 5 µg/ml.|
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Treatement for 30 minutes (lanes 1-4) or for 1 hour (lanes 5-8). 12.5% SDS-PAGE. Proteins collected in RIPA Buffer and transferred to PVDF (0.45 um).