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Synthetic peptide derived from the sequence of Mouse Bad, conjugated to KLH.
Our Abpromise guarantee covers the use of ab90435 in the following tested applications.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa.|
|IHC-P||1/50. Antigen retrieval is not essential but may optimise staining.|
|ICC/IF||Use a concentration of 5 µg/ml.|
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Treatement for 30 minutes (lanes 1-4) or for 1 hour (lanes 5-8). 12.5% SDS-PAGE. Proteins collected in RIPA Buffer and transferred to PVDF (0.45 um).