- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-BAF53A antibody
See all BAF53A primary antibodies -
DescriptionGoat polyclonal to BAF53A
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Specificityab15718 is expected to recognise both BAF53A and BAF53B, which are almost identical.
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide:
YEEGGKQCVERKCP
, corresponding to C terminal amino acids 416-429 of BAF53A. -
Positive control
- HeLa lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab15718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human BAF53A peptide (ab23271). |
Target
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RelevanceThis protein is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This protein is a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. It is required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 86 Human
- Entrez Gene: 56456 Mouse
- Omim: 604958 Human
- SwissProt: O96019 Human
- SwissProt: Q9Z2N8 Mouse
- Unigene: 435326 Human
- Unigene: 41077 Mouse
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Alternative names
- 53 kDa BRG1 associated factor A antibody
- Actin like 6A antibody
- Actin like protein 6A antibody
see all
Protocols
References for Anti-BAF53A antibody (ab15718)
ab15718 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"