• Product nameAnti-BAF53A antibody
    See all BAF53A primary antibodies
  • Description
    Rabbit polyclonal to BAF53A
  • Specificityab64989 detects endogenous levels of total BAF53A protein.
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from internal sequence of human BAF53A.

  • Positive control
    • Extracts from Jurkat cells and COLO205 cells. HUVEC cells.


Associated products


Our Abpromise guarantee covers the use of ab64989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.


  • RelevanceThis protein is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This protein is a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. It is required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • 53 kDa BRG1 associated factor A antibody
    • Actin like 6A antibody
    • Actin like protein 6A antibody
    • Actin related protein antibody
    • Actin related protein Baf53a antibody
    • actin-related protein 4 antibody
    • ACTL 6 antibody
    • ACTL 6A antibody
    • ACTL6 antibody
    • ACTL6A antibody
    • Arp4 antibody
    • ARPN BETA antibody
    • ArpNbeta antibody
    • BAF 53 antibody
    • BAF 53A antibody
    • BAF complex 53 kDa subunit antibody
    • BAF53 antibody
    • BRG1 associated factor 53A antibody
    • BRG1 associated factor antibody
    • BRG1/brm associated factor 53A antibody
    • hArpN beta antibody
    • INO80 complex subunit K antibody
    • INO80K antibody
    • MGC5382 antibody
    see all

Anti-BAF53A antibody images

  • All lanes : Anti-BAF53A antibody (ab64989) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells with no immunising peptide
    Lane 2 : Extracts from COLO205 cells with no immunising peptide
    Lane 3 : Extracts from COLO205 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • Immunofluorescence analysis of HUVEC cells, using BAF53A antibody (ab64989) at 1/500 - 1/1000 dilution, in the presence (right panel) and absence (left panel) of immunising peptide.

References for Anti-BAF53A antibody (ab64989)

ab64989 has not yet been referenced specifically in any publications.

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