Overview

  • Product name
    Anti-BAF57/SMARCE1 antibody
    See all BAF57/SMARCE1 primary antibodies
  • Description
    Goat polyclonal to BAF57/SMARCE1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    PPTDPIPEDEKK

    with a Cysteine residue linker, corresponding to C terminal amino acids 399-410 of Human BAF57/SMARCE1 (NP_003070.3).

  • Positive control
    • Human prostate tissue; Jurkat lysate; HEK293 lysate overexpressing BAF57/SMARCE1.

Properties

Applications

Our Abpromise guarantee covers the use of ab115512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 47 kDa.
ELISA 1/16000.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Domain
    The HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAF57 antibody
    • BRG1 associated factor 57 antibody
    • BRG1-associated factor 57 antibody
    • Chromatin remodeling complex BRG1 associated factor 57 antibody
    • FLJ35648 antibody
    • SMARCE 1 antibody
    • SMARCE1 antibody
    • SMCE1_HUMAN antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin e1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 antibody
    see all

Images

  • ab115512 at 3.75ug/ml staining BAF57/SMARCE1 in Human prostate tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-BAF57/SMARCE1 antibody (ab115512) at 0.2 µg/ml + Jurkat lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 47 kDa
  • All lanes : Anti-BAF57/SMARCE1 antibody (ab115512) at 0.6 µg/ml

    Lane 1 : HEK293 lysate, mock transfection control
    Lane 2 : HEK293 lysate, overexpressing BAF57/SMARCE1


    Predicted band size : 47 kDa

References

ab115512 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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