Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab8396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 34 kDa.Can be blocked with BAFF peptide (ab8397).

Target

  • FunctionCytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
  • Tissue specificityAbundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    N-glycosylated.
  • Cellular localizationSecreted and Cell membrane.
  • Target information above from: UniProt accession Q9Y275 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ApoL related ligand TALL 1 antibody
    • B cell Activating Factor antibody
    • B cell Activating Factor antibody
    • B lymphocyte stimulator antibody
    • B-cell-activating factor antibody
    • BAFF antibody
    • BLYS antibody
    • CD 257 antibody
    • CD257 antibody
    • CD257 antigen antibody
    • delta BAFF antibody
    • Dendritic cell derived TNF like molecule antibody
    • Dendritic cell-derived TNF-like molecule antibody
    • DTL antibody
    • DTL precursor antibody
    • PRO738 antibody
    • soluble form antibody
    • TALL 1 antibody
    • TALL-1 antibody
    • TALL1 antibody
    • THANK antibody
    • TN13B_HUMAN antibody
    • TNF and APOL related leukocyte expressed ligand 1 antibody
    • TNF homolog that activates apoptosis antibody
    • TNF homolog that activates apoptosis NKFB and JNK. antibody
    • TNF- and APOL-related leukocyte expressed ligand 1 antibody
    • TNFSF13B antibody
    • TNFSF20 antibody
    • Tumor necrosis factor ligand superfamily member 13B antibody
    • Tumor necrosis factor ligand superfamily member 20 antibody
    • Tumor necrosis factor like protein ZTNF4 antibody
    • Tumor necrosis factor superfamily member 13B antibody
    • UNQ401 antibody
    • ZTNF4 antibody
    see all

Anti-BAFF antibody images

  • All lanes : Anti-BAFF antibody (ab8396) at 1 µg/ml

    Lane 1 : Human HL60 cell lysate
    Lane 2 : Murine spleen tissue lysate


    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • ab8396 at 1µg/ml staining BAFF in HL60 cells by ICC/IF

References for Anti-BAFF antibody (ab8396)

ab8396 has not yet been referenced specifically in any publications.

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Thank you for your inquiry. The ab8396 can only be used for blocking. Indeed, the peptide is very short containing only 16 amino acids. I would therefore not recommend it for using as a control by loading on the gel. It would run at around 1.8kD - whic...

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Thank you for your enquiry. To our knowledge, neither of these antibodies have yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"