Anti-Bag1 antibody - Aminoterminal end (ab45411)


  • Product nameAnti-Bag1 antibody - Aminoterminal end
    See all Bag1 primary antibodies
  • Description
    Rabbit polyclonal to Bag1 - Aminoterminal end
  • SpecificityThis antibody is specific for Bag1.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 14 amino acid peptide from near the amino terminus of human Bag 1.

  • Positive control
    • PC-3 cell lysate.


Our Abpromise guarantee covers the use of ab45411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).


  • FunctionInhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
  • Tissue specificityIsoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
  • Sequence similaritiesContains 1 BAG domain.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bag 1 antibody
    • BAG family molecular chaperone regulator 1 antibody
    • BAG-1 antibody
    • BAG1 antibody
    • BAG1_HUMAN antibody
    • BCL 2 Associated Athanogene 1 antibody
    • BCL 2 associated athanogene antibody
    • BCL 2 binding athanogene 1 antibody
    • Bcl-2-associated athanogene 1 antibody
    • BCL2 associated athanogene 1 antibody
    • BCL2 associated athanogene antibody
    • Glucocorticoid receptor-associated protein RAP46 antibody
    • HAP1 antibody
    • Haptoglobin antibody
    • RAP 46 antibody
    • RAP46 antibody
    see all

Anti-Bag1 antibody - Aminoterminal end images

  • Lane 1 : Anti-Bag1 antibody - Aminoterminal end (ab45411) at 1 µg/ml
    Lane 2 : Anti-Bag1 antibody - Aminoterminal end (ab45411) at 2 µg/ml

    Lane 1 : PC-3 cell lysate
    Lane 2 : PC-3 cell lysate

    Predicted band size : 31 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab45411 at 2µg/ml staining Bag1-Aminoterminal end in human lymph nodes by IHC

  • Immunofluorescence of BAG-1 in Human Lymph Node cells using ab45411 at 20 ug/ml.

References for Anti-Bag1 antibody - Aminoterminal end (ab45411)

ab45411 has not yet been referenced specifically in any publications.

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