Bag1 antibody [Y166] (ab32109)

Product Name 

Bag1 antibody [Y166]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [Y166] to Bag1

Immunogen 

A synthetic peptide corresponding to residues following the repeat 7 of human Bag-1.

Reacts with 

Hu, Rat

Does not react with 
(species key)

Ms

Specificity 

The antibody should recognize all three isoforms of Bag-1 (Bag-1L, Bag-1M and Bag-1S).

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/100 - 1/250.
IHC-P: 1/100 - 1/250.
IP: 1/80.
WB: 1/500. Detects bands of approximately 33, 46 and 50 kDa (predicted molecular weight: 31 kDa).

Is unsuitable for FACS.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysate and cells, human prostate carcinoma tissue

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cardiovascular >> Heart >> Autophagy >> Autophagosome
Cardiovascular >> Atherosclerosis >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family

Relevance 

Bag1 is a multifunctional protein that blocks apoptosis and interacts with several types of proteins. These include the Bcl 2 family proteins, the kinase Raf 1, certain tyrosine kinase growth factor receptors, and steroid hormone receptors, possibly by virtue of its ability to regulate the Hsp70 / Hsc70 family of molecular chaperones. Bag1 binds the ATPase domains of Hsp70 and Hsc70, modulating their chaperone activity and functioning as a competitive antagonist of the co chaperone Hip. Human BAG 1 (hBAG 1) cDNA is identical to a hormone receptor binding protein RAP46. Human Bag1 protein displays different isoforms of 36 kDa, 46 kDa, and 50 kDa.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

Y166

Isotype 

IgG

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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