General notesab94217 is a 293T cell transfected lysate in which Human Bag1 has been transiently over-expressed using a pCMV-Bag1 plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more detailed how the transfected lysate was prepared view preparation notes
BackgroundFunction: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
Tissue specificity: Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
Similarity: Contains 1 BAG domain.
Contains 1 ubiquitin-like domain.
PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.