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Western blot - BAG2 antibody (ab752)
(enlarge)
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Product Name
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BAG2 antibody
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See all BAG2 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to BAG2
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Immunogen
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Synthetic peptide: SKTLQQNAESRFN, corresponding to C terminal amino acids 199-211 of Human BAG2
BLAST 'SKTLQQNAESRFN' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: 1/16000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 24 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate.
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Relevance
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BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: Tris saline, 0.5% BSA. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BAG2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BAG2 antibody images, references, products related to ab752 and other tools.
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BAG2 antibody images:
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 Western blot - BAG2 antibody (ab752)
BAG2 antibody (ab752) at 0.3 µg/ml + Hela lysate (35ug protein in RIPA buffer).
Predicted band size : 24 kDa
Observed band size : 24 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
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Search PubMed (MEDLINE) for references to BAG2
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BAG2 antibody - more information
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Customer reviews (feedback) regarding BAG2 antibody |
Customer FAQs regarding BAG2 antibody |
Protocols for BAG2 antibody |
Price and availability of products related to BAG2 antibody |
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BAG2 antibody - related products:
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BAG2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about BAG2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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