Overview

  • Product name
    Anti-BAG2 antibody [EPR3567]
    See all BAG2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3567] to BAG2
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human BAG2 aa 1-100 (N terminal).

  • Positive control
    • Jurkat, HeLa and HepG2 cell lysates; human ovarian carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab79406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IP 1/10.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.
  • Sequence similarities
    Contains 1 BAG domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAG 2 antibody
    • BAG family molecular chaperone regulator 2 antibody
    • BAG-2 antibody
    • BAG-family molecular chaperone regulator-2 antibody
    • Bag2 antibody
    • BAG2_HUMAN antibody
    • BCL 2 associated athanogene 2 antibody
    • Bcl-2-associated athanogene 2 antibody
    • BCL2 associated athanogene 2 antibody
    • BCL2-associated athanogene 2 antibody
    • dJ417I1.2 (BAG family molecular chaperone regulator 2) antibody
    • dJ417I1.2 antibody
    • KIAA0576 antibody
    • MGC149462 antibody
    • OTTHUMP00000016668 antibody
    see all

Images

  • All lanes : Anti-BAG2 antibody [EPR3567] (ab79406) at 1/5000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
  • ab79406, at 1/100 dilution, staining BAG2 in human ovarian carcinoma by Immunohistochemistry using paraffin-embedded tissue.

References

This product has been referenced in:
  • Azoitei N  et al. Targeting of KRAS mutant tumors by HSP90 inhibitors involves degradation of STK33. J Exp Med 209:697-711 (2012). WB . Read more (PubMed: 22451720) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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