Recombinant
RabMAb

Anti-BAG2 antibody [EPR3567] (Alexa Fluor® 568) (ab212105)

Overview

  • Product name
    Anti-BAG2 antibody [EPR3567] (Alexa Fluor® 568)
    See all BAG2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3567] to BAG2 (Alexa Fluor® 568)
  • Conjugation
    Alexa Fluor® 568. Ex: 578nm, Em: 603nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BAG2 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O95816

  • Positive control
    • ICC/IF: HeLa cells.
  • General notes

     

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

Properties

Applications

Our Abpromise guarantee covers the use of ab212105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function
    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.
  • Sequence similarities
    Contains 1 BAG domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAG 2 antibody
    • BAG family molecular chaperone regulator 2 antibody
    • BAG-2 antibody
    • BAG-family molecular chaperone regulator-2 antibody
    • Bag2 antibody
    • BAG2_HUMAN antibody
    • BCL 2 associated athanogene 2 antibody
    • Bcl-2-associated athanogene 2 antibody
    • BCL2 associated athanogene 2 antibody
    • BCL2-associated athanogene 2 antibody
    • dJ417I1.2 (BAG family molecular chaperone regulator 2) antibody
    • dJ417I1.2 antibody
    • KIAA0576 antibody
    • MGC149462 antibody
    • OTTHUMP00000016668 antibody
    see all

Images

  • ab212105 staining BAG2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab212105 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

     

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

References

ab212105 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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