Overview

  • Product nameAnti-Bag3 antibody
    See all Bag3 primary antibodies
  • Description
    Rabbit polyclonal to Bag3
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A recombinant protein fragment corresponding to the C terminal 196 amino acids of human BAG3.

  • Positive control
    • Muscle, PANC-1 pancreatic cell line (ATCC-CRL 1469)

Properties

Applications

Our Abpromise guarantee covers the use of ab47124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/1000 - 1/5000.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Predicted molecular weight: 62 kDa.
IP 1/50 - 1/200.

Target

  • FunctionInhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
  • Involvement in diseaseDefects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
  • Sequence similaritiesContains 1 BAG domain.
    Contains 2 WW domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAG 3 antibody
    • BAG family molecular chaperone regulator 3 antibody
    • BAG-3 antibody
    • Bag3 antibody
    • BAG3_HUMAN antibody
    • Bcl 2 binding protein antibody
    • Bcl-2-associated athanogene 3 antibody
    • Bcl-2-binding protein Bis antibody
    • BCL2 associated athanogene 3 antibody
    • BCL2 binding athanogene 3 antibody
    • BIS antibody
    • CAIR 1 antibody
    • Docking protein CAIR 1 antibody
    • Docking protein CAIR-1 antibody
    • MFM6 antibody
    see all

Anti-Bag3 antibody images

  • All lanes : Anti-Bag3 antibody (ab47124) at 1/2000 dilution

    Lane 1 : Thymus lysates, total protein (20ug/lane)
    Lane 2 : Spleen lysates, total protein (20ug/lane)
    Lane 3 : Skeletal(quadriceps) lysates, total protein (20ug/lane)
    Lane 4 : Skeletal(diaphragm) lysates, total protein (20ug/lane)
    Lane 5 : Smooth (heart) muscle lysates, total protein (20ug/lane)


    Predicted band size : 62 kDa
    Observed band size : 86 kDa (why is the actual band size different from the predicted?)
  • Frozen mouse muscle tissue section stained for BAG3 expression using ab47124 at 1/2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins."
  • Immunohistochemistry of formalin fixed paraffin embedded human pancreas tissue sections using ab47124 at 1/2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.
  • ICC/IF image of ab47124 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47124, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Bag3 antibody (ab47124)

This product has been referenced in:
  • Pasillas MP  et al. Proteomic analysis reveals a role for Bcl2-associated athanogene 3 and major vault protein in resistance to apoptosis in senescent cells by regulating ERK1/2 activation. Mol Cell Proteomics 14:1-14 (2015). Read more (PubMed: 24997994) »
  • Boiani M  et al. The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737. J Biol Chem 288:6980-90 (2013). WB ; Human . Read more (PubMed: 23341456) »

See all 7 Publications for this product

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