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Product Name
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Bag3 antibody
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See all Bag3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Bag3
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Note on antibody grade |
Fast-Track antibodies
- Immunogen affinity purified
- high ELISA titre values
- released early (to accelerate your research)
- 950 Abpoints to the 1st researcher
who submits conclusive data (positive or negative):
Read the Fast-Track antibody Terms of Use
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Immunogen
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Synthetic peptide: SSMTDTPGNPAAP, corresponding to C terminal amino acids 563-575 of Human Bag3.
BLAST 'C-SSMTDTPGNPAAP' with  or 
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Reacts with
(species key)
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Hu
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Peptide ELISA: antibody detection limit dilution 1:8,000.
Western Blot: Preliminary experiments gave an approx 80kDa band in Hela and 293 lysates at 1ug of primary. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 67Kda according to NP_004272.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Relevance
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BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45 amino acid BAG domain near the C terminus but differ markedly in their N terminal regions. The protein encoded by this gene contains a WW domain in the N terminal region and a BAG domain in the C terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip repressible manner.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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GenBank Accession Number – NP_004272
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bag3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Bag3
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Bag3 antibody - more information
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Customer reviews (feedback) regarding Bag3 antibody |
Customer FAQs regarding Bag3 antibody |
Protocols for Bag3 antibody |
Price and availability of products related to Bag3 antibody |
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Bag3 antibody - related products:
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Bag3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Bag3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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