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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bag3 antibody
See all Bag3 products (7) ...
Description
Goat polyclonal to Bag3
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: SSMTDTPGNPAAP, corresponding to C terminal amino acids 563-575 of Human Bag3.
C-SSMTDTPG NPAAP
Positive control
Human skeletal muscle lysates.
General notes
GenBank Accession Number – NP_004272
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.3 µg/ml.(Approx 85kDa band observed in Human skeletal muscle lysates (calculated MW of 61.6kDa according to NP_004272.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Iwasaki et al, Cancer Res. 2007 Nov 1;67(21):10252-9. PMID: 17974966).)
Target
Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
Involvement in disease
Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
Sequence similarities
Contains 1 BAG domain.
Contains 2 WW domains.
Target information above from: UniProt accessionO95817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BAG 3 antibody
- BAG family molecular chaperone regulator 3 antibody
- BAG family molecular chaperone regulator 3 antibody
see all
Database links
- Entrez Gene: 9531 Human
- Omim: 603883 Human
- SwissProt: O95817 Human
- Unigene: 15259 Human
- Unigene: 523309 Human
References for Anti-Bag3 antibody (ab5898)
This product has been referenced in:
- Zimmermann Ket al. Transient receptor potential cation channel, subfamily C, member 5 (TRPC5) is a cold-transducer in the peripheral nervous system. Proc Natl Acad Sci U S A 108:18114-9 (2011).Read more (PubMed: 22025699) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"