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Synthetic peptide: SSMTDTPGNPAAP, corresponding to C terminal amino acids 563-575 of Human Bag3.
Our Abpromise guarantee covers the use of ab5898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 23341456|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Can be blocked with Human Bag3 peptide (ab23111).
Approx 85kDa band observed in Human skeletal muscle lysates (calculated MW of 61.6kDa according to NP_004272.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Iwasaki et al, Cancer Res. 2007 Nov 1;67(21):10252-9. PMID: 17974966).
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