• Product name
  • Description
    Rabbit polyclonal to Bag3
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Bag3.

    (Peptide available as ab96631.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human lung; Human heart; Human spleen;



Our Abpromise guarantee covers the use of ab86298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa).
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
  • Involvement in disease
    Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
  • Sequence similarities
    Contains 1 BAG domain.
    Contains 2 WW domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAG 3 antibody
    • BAG family molecular chaperone regulator 3 antibody
    • BAG-3 antibody
    • Bag3 antibody
    • BAG3_HUMAN antibody
    • Bcl 2 binding protein antibody
    • Bcl-2-associated athanogene 3 antibody
    • Bcl-2-binding protein Bis antibody
    • BCL2 associated athanogene 3 antibody
    • BCL2 binding athanogene 3 antibody
    • BIS antibody
    • CAIR 1 antibody
    • Docking protein CAIR 1 antibody
    • Docking protein CAIR-1 antibody
    • MFM6 antibody
    see all


  • All lanes : Anti-Bag3 antibody (ab86298) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 minute
  • ICC/IF image of ab86298 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86298 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Hek293, HepG2, and MCF-7 cells in PFA at 5ug/ml, and in HeLa, Hek293, HepG2, MCF-7 cells in Methanol at 5ug/ml.

  • IHC image of Ab86298 staining in Humna Cervical Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab86298, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Inomata M  et al. Regulation of Toll-like receptor signaling by NDP52-mediated selective autophagy is normally inactivated by A20. Cell Mol Life Sci 69:963-79 (2012). Read more (PubMed: 21964925) »

See 1 Publication for this product

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Mouse Cell (Primary Hepatocytes)
Primary Hepatocytes

Abcam user community

Verified customer

Submitted Jan 23 2014

Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Mouse Cell lysate - whole cell (primary hepatoctyes)
primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 02 2013


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