Overview

  • Product name
    Anti-Bag3 antibody [EPR3515]
    See all Bag3 primary antibodies
  • Description
    Rabbit monoclonal [EPR3515] to Bag3
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Bag3.

  • Positive control
    • 293T, HeLa, MCF7 and K562 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 24895585
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
    • Involvement in disease
      Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
    • Sequence similarities
      Contains 1 BAG domain.
      Contains 2 WW domains.
    • Information by UniProt
    • Database links
    • Alternative names
      • BAG 3 antibody
      • BAG family molecular chaperone regulator 3 antibody
      • BAG-3 antibody
      • Bag3 antibody
      • BAG3_HUMAN antibody
      • Bcl 2 binding protein antibody
      • Bcl-2-associated athanogene 3 antibody
      • Bcl-2-binding protein Bis antibody
      • BCL2 associated athanogene 3 antibody
      • BCL2 binding athanogene 3 antibody
      • BIS antibody
      • CAIR 1 antibody
      • Docking protein CAIR 1 antibody
      • Docking protein CAIR-1 antibody
      • MFM6 antibody
      see all

    Images

    • All lanes : Anti-Bag3 antibody [EPR3515] (ab92309) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 62 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Xiao H  et al. BAG3 and HIF-1 a Coexpression Detected by Immunohistochemistry Correlated with Prognosis in Hepatocellular Carcinoma after Liver Transplantation. Biomed Res Int 2014:516518 (2014). WB, IHC-P ; Human . Read more (PubMed: 24895585) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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