The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 24895585
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
1/10 - 1/100.
Is unsuitable for Flow Cyt or ICC.
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
Involvement in disease
Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
BAG family molecular chaperone regulator 3 antibody
Bcl 2 binding protein antibody
Bcl-2-associated athanogene 3 antibody
Bcl-2-binding protein Bis antibody
BCL2 associated athanogene 3 antibody
BCL2 binding athanogene 3 antibody
CAIR 1 antibody
Docking protein CAIR 1 antibody
Docking protein CAIR-1 antibody
Western blot - Anti-Bag3 antibody [EPR3515] (ab92309)
Predicted band size : 62 kDa
Lane 1: Wild-type HAP1 whole cell lysate (20 µg) Lane 2: BAG3 knockout HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg) Lane 4: K562 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab92309 observed at 80 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab92309 was shown to recognize BAG3 in wild-type cells as signal was lost at the expected MW in BAG3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BAG3 knockout samples were subjected to SDS-PAGE. Ab92309 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Bag3 antibody [EPR3515] (ab92309)
All lanes : Anti-Bag3 antibody [EPR3515] (ab92309) at 1/1000 dilution
Lane 1 : 293T cell
lysate Lane 2 : HeLa cell
lysate Lane 3 :
MCF7 cell lysate Lane 4 : K562 cell lysate
Xiao H et al. BAG3 and HIF-1 a Coexpression Detected by Immunohistochemistry Correlated with Prognosis in Hepatocellular Carcinoma after Liver Transplantation. Biomed Res Int2014:516518 (2014).
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