The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.
Use at an assay dependent concentration. PubMed: 24475098
FunctionInhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding.
Sequence similaritiesContains 5 BAG domains.
DomainThe fifth BAG domain is responsible for the interaction with HSP70 nucleotide-binding domain.
BAG family molecular chaperone regulator 5 antibody
Bcl 2 associated athanogene 5 antibody
Bcl-2-associated athanogene 5 antibody
BCL2 associated athanogene 5 antibody
Anti-Bag5 antibody images
Western blot - Bag5 antibody (ab56738)
Predicted band size : 51 kDa Bag5 antibody (ab56738) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
References for Anti-Bag5 antibody (ab56738)
This product has been referenced in:
Wang X et al. BAG5 protects against mitochondrial oxidative damage through regulating PINK1 degradation. PLoS One9:e86276 (2014).
Read more (PubMed: 24475098) »
Boiani M et al. The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737. J Biol Chem288:6980-90 (2013).
Read more (PubMed: 23341456) »