Overview

  • Product nameAnti-Bag5 antibody
    See all Bag5 primary antibodies
  • Description
    Mouse polyclonal to Bag5
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human Bag5 (NP_004864.1).

  • Positive control
    • WB: Human spleen tissue lysate; HeLa cell lysate; Bag5 transfected 293T cell lysate ICc/IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab88100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionInhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding.
  • Sequence similaritiesContains 5 BAG domains.
  • DomainThe fifth BAG domain is responsible for the interaction with HSP70 nucleotide-binding domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAG family molecular chaperone regulator 5 antibody
    • BAG-5 antibody
    • BAG5 antibody
    • BAG5_HUMAN antibody
    • Bcl 2 associated athanogene 5 antibody
    • Bcl-2-associated athanogene 5 antibody
    • BCL2 associated athanogene 5 antibody
    • KIAA0873 antibody
    see all

Anti-Bag5 antibody images

  • Anti-Bag5 antibody (ab88100) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size : 51 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
  • Anti-Bag5 antibody (ab88100) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • All lanes : Anti-Bag5 antibody (ab88100) at 1 µg/ml

    Lane 1 : Bag5 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate


    Predicted band size : 51 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • ab88100, at 10 µg/ml, staining Bag5 in HeLa cell by Immunofluorescence.

References for Anti-Bag5 antibody (ab88100)

ab88100 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"