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Rabbit polyclonal to Bag5
Predicted to work with:
Mouse, Rat, Cow
Recombinant fragment, corresponding to a sequence within amino acids 1-165 of Human Bag5 (NP_004864).
293T, HeLa, MOLT4 and Raji cell lysates; A549 cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
1/100 - 1/200.
Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding.
Contains 5 BAG domains.
The fifth BAG domain is responsible for the interaction with HSP70 nucleotide-binding domain.
Information by UniProt
BAG family molecular chaperone regulator 5 antibody
Western blot - Anti-Bag5 antibody (ab97660)
All lanes : Anti-Bag5 antibody (ab97660) at 1/1000 dilution Lane 1 : HeLa whole cell lysate Lane 2 : MOLT4 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 51 kDa 10% SDS-PAGE.
Immunocytochemistry/ Immunofluorescence - Anti-Bag5 antibody (ab97660)
ab97660 at 1/200 dilution staining Bag5 in A549 cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"