General notesab94322 is a 293T cell transfected lysate in which Human Bag5 has been transiently over-expressed using a pCMV-Bag5 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
BackgroundFunction: Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding.
Similarity: Contains 5 BAG domains.
Domain: The fifth BAG domain is responsible for the interaction with HSP70 nucleotide-binding domain.