Bag5 overexpression 293T lysate (whole cell) (ab94322)

Overview

  • Product nameBag5 overexpression 293T lysate (whole cell)
  • Description
    Bag5 overexpression lysate
  • General notesab94322 is a 293T cell transfected lysate in which Human Bag5 has been transiently over-expressed using a pCMV-Bag5 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding. Similarity: Contains 5 BAG domains. Domain: The fifth BAG domain is responsible for the interaction with HSP70 nucleotide-binding domain.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Bag5 overexpression 293T lysate (whole cell) images

  • ab94322 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Bag5 antibody (ab88100) at 1/500 dilution

    Lane 1 : Bag5 overexpression 293T lysate (whole cell) (ab94322)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Bag5 overexpression 293T lysate (whole cell) (ab94322)

ab94322 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"