This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

  • Product name
  • Description
    Rabbit polyclonal to BAHD1

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species
    Rabbit
  • Species reactivity

    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human BAHD1.

    (Peptide available as ab66437.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa, Jurkat and HEK293

Properties

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB
  • Application notes
    This antibody gave a positive result in ELISA against the immunizing peptide (ab66437).
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      BAHD1 (bromo adjacent homology domain-containing 1 protein) is, as its name suggests, a bromo-adjacent homology (BAH) domain. The BAH domain is found in eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, and several proteins involved in transcriptional regulation. It is thought to function as a protein-protein interaction domain. The BAH domain of yeast Sir3p has been shown to bind to nucleosomes and to the N-terminal tail of histone H3.
    • Cellular localization
      Nucleus. Note: Localizes to heterochromatin and inactive X chromosome. Colocalizes with histone H3 trimethylated at 'Lys-27' (H3K27me3).
    • Database links
    • Alternative names
      • AL022997 antibody
      • AW541238 antibody
      • BAH domain containing protein 1 antibody
      • BAH domain containing protein1 antibody
      • BAHDH 1 antibody
      • Bromo adjacent homology domain containing 1 antibody
      • Bromo adjacent homology domain containing1 antibody
      • Bromo adjacent homology domain-containing 1 protein antibody
      • Gm117 antibody
      • KIAA0945 antibody
      • MGC117747 antibody
      • mKIAA0945 antibody
      • RGD1563127 antibody
      see all

    Images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • All lanes : Anti-BAHD1 antibody (ab46573) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 85 kDa
      Observed band size: 75 kDa (why is the actual band size different from the predicted?)



      ab46573 detects a band at 75 kDa in a range of human cell lysates. We are unsure as to why the band seen is lower than predicted and would welcome any customer feedback and comments regarding this product and the data presented above.

    References

    This product has been referenced in:
    • Libertini E  et al. Overexpression of the Heterochromatinization Factor BAHD1 in HEK293 Cells Differentially Reshapes the DNA Methylome on Autosomes and X Chromosome. Front Genet 6:339 (2015). WB, ICC/IF ; Human . Read more (PubMed: 26648976) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab46573.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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