This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

Applications

  • Application notes
    This antibody gave a positive result in ELISA against the immunizing peptide (ab66437).
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      BAHD1 (bromo adjacent homology domain-containing 1 protein) is, as its name suggests, a bromo-adjacent homology (BAH) domain. The BAH domain is found in eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, and several proteins involved in transcriptional regulation. It is thought to function as a protein-protein interaction domain. The BAH domain of yeast Sir3p has been shown to bind to nucleosomes and to the N-terminal tail of histone H3.
    • Cellular localization
      Nucleus. Note: Localizes to heterochromatin and inactive X chromosome. Colocalizes with histone H3 trimethylated at 'Lys-27' (H3K27me3).
    • Database links
    • Alternative names
      • AL022997 antibody
      • AW541238 antibody
      • BAH domain containing protein 1 antibody
      • BAH domain containing protein1 antibody
      • BAHDH 1 antibody
      • Bromo adjacent homology domain containing 1 antibody
      • Bromo adjacent homology domain containing1 antibody
      • Bromo adjacent homology domain-containing 1 protein antibody
      • Gm117 antibody
      • KIAA0945 antibody
      • MGC117747 antibody
      • mKIAA0945 antibody
      • RGD1563127 antibody
      see all

    Images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • All lanes : Anti-BAHD1 antibody (ab46573) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 85 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Libertini E  et al. Overexpression of the Heterochromatinization Factor BAHD1 in HEK293 Cells Differentially Reshapes the DNA Methylome on Autosomes and X Chromosome. Front Genet 6:339 (2015). WB, ICC/IF ; Human . Read more (PubMed: 26648976) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab46573.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up