Anti-BAI1 antibody (ab135907)
Key features and details
- Rabbit polyclonal to BAI1
- Suitable for: IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BAI1 antibody -
Description
Rabbit polyclonal to BAI1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human BAI1 aa 683-732 (internal sequence).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49.1% PBS, 0.88% Sodium chloride
PBS without Mg2+ or Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit anti-serum by immunogenic peptide affinity-chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab135907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 24509909
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 174 kDa.
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IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 24509909 |
WB
1/500 - 1/1000. Predicted molecular weight: 174 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Likely to be a potent inhibitor of angiogenesis in brain and may play a significant role as a mediator of the p53 signal in suppression of glioblastoma. May function in cell adhesion and signal transduction in the brain. -
Tissue specificity
Specifically expressed in brain. Reduced or no expression is observed in some glioblastoma cell lines and cancer tissues. No expression in astrocytes. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 1 GPS domain.
Contains 5 TSP type-1 domains. -
Domain
The TSP1 repeats inhibit in vivo angiogenesis in rat cornea induced by BFGF. -
Post-translational
modificationsThe endogenous protein is proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit. -
Cellular localization
Cell membrane. Likely to be concentrated at cell-cell adhesion sites. - Information by UniProt
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Database links
- Entrez Gene: 575 Human
- Entrez Gene: 107831 Mouse
- Omim: 602682 Human
- SwissProt: O14514 Human
- SwissProt: Q3UHD1 Mouse
- Unigene: 194654 Human
- Unigene: 43133 Mouse
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Alternative names
- B830018M07Rik antibody
- BAI 1 antibody
- BAI1 antibody
see all
Images
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Anti-BAI1 antibody (ab135907) at 1/500 dilution + Huvec cell lysate at 30 µg
Predicted band size: 174 kDa -
Immunohistochemical analysis of paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized paraffin-embedded mouse hippocampus tissue labeling BAI1 with ab135907 at 1/50 dilution,followed by Goat Anti-Rabbit IgG (HRP) at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab135907 has been referenced in 6 publications.
- An HJ et al. BAI1 nuclear expression reflects the survival of nonsmoking non-small cell lung cancer patients. Thorac Cancer 12:1673-1680 (2021). PubMed: 33934543
- Benavente F et al. Novel C1q receptor-mediated signaling controls neural stem cell behavior and neurorepair. Elife 9:N/A (2020). PubMed: 32894219
- Tian DW et al. Biological effects of eukaryotic recombinant plasmid pReceiver-M61-BAI-1 transfection on T24 cells and HUVECs. Mol Med Rep 14:1553-9 (2016). Human . PubMed: 27356780
- Tian D et al. Expression of brain-specific angiogenesis inhibitor-1 and association with p53, microvessel density and vascular endothelial growth factor in the tissue of human bladder transitional cell carcinoma. Mol Med Rep 12:4522-4529 (2015). PubMed: 26129954
- Belone Ade F et al. Genome-Wide Screening of mRNA Expression in Leprosy Patients. Front Genet 6:334 (2015). IHC ; Human . PubMed: 26635870
- Das S et al. Brain angiogenesis inhibitor 1 is expressed by gastric phagocytes during infection with Helicobacter pylori and mediates the recognition and engulfment of human apoptotic gastric epithelial cells. FASEB J 28:2214-24 (2014). IHC-Fr ; Human . PubMed: 24509909