Anti-BAIAP2 antibody (ab37542)

Overview

  • Product name
  • Description
    Rabbit polyclonal to BAIAP2
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide (10-30 aa in length) at the C terminal of last 50 aa of Human BAIAP2.

  • Positive control
    • Mouse brain tissue lysate, A375 cell lysate and Human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ELISA 1/1000.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation.
  • Tissue specificity
    Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta.
  • Sequence similarities
    Contains 1 IMD (IRSp53/MIM homology) domain.
    Contains 1 SH3 domain.
  • Domain
    The IMD domain forms a coiled coil. The isolated domain can induce actin bundling and filopodia formation. In the absence of G-proteins intramolecular interaction between the IMD and the SH3 domain gives rise to an auto-inhibited state of the protein. Interaction of the IMD with RAC1 or CDC42 leads to activation.
    The SH3 domain interacts with ATN1, BAI1, WASF1, WASF2, SHANK1, DIAPH1 and ENAH.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues by INSR in response to insulin treatment.
  • Cellular localization
    Cytoplasm. Membrane. Cell projection > filopodium. Cell projection > ruffle. Detected throughout the cytoplasm in the absence of specific binding partners. Detected in filopodia and close to membrane ruffles.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAI-associated protein 2 antibody
    • BAI1 associated protein 2 antibody
    • BAI1-associated protein 2 antibody
    • BAIAP 2 antibody
    • Baiap2 antibody
    • BAIP2_HUMAN antibody
    • BAP2 antibody
    • Brain-specific angiogenesis inhibitor 1-associated protein 2 antibody
    • Fas ligand-associated factor 3 antibody
    • FLAF3 antibody
    • Insulin receptor substrate p53 antibody
    • Insulin receptor substrate p53/p58 antibody
    • Insulin receptor substrate protein of 53 kDa antibody
    • IRS-58 antibody
    • IRSp53 antibody
    • IRSp53/58 antibody
    • Protein BAP2 antibody
    see all

Anti-BAIAP2 antibody images

  • All lanes : Anti-BAIAP2 antibody (ab37542) at 1/100 dilution

    Lane 1 : Mouse brain cell lysate
    Lane 2 : A375 cell lysate


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • Formalin-fixed and paraffin-embedded Human breast cancer tissue reacted with ab37542, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

References for Anti-BAIAP2 antibody (ab37542)

ab37542 has not yet been referenced specifically in any publications.

Product Wall

I have contacted the lab who have agreed to add the blocking peptide for ab37542 to the catalogue. The product is called BAIAP2 peptide, ab170411.

As for EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, I can confirm that it does h...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human HEK293 cell)
Loading amount
20 µg
Specification
human HEK293 cell
Gel Running Conditions
Reduced Denaturing (8% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Mar 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01 M Tris, pH 10
Permeabilization
Yes - 0.3% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Loading amount
20 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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