Cell-permeable flavonoid. Lipoxygenase inhibitor (IC50 values are 0.64 and 1.6 μM at 12- and 15-lipoxygenases, respectively). Shows antioxidant and anti-inflammatory properties. Also induces apoptosis. Additionally, inhibits α-syn oligomerisation, β-Amyloid induced neurotoxicity and amyloid protein aggregation.
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Soluble in DMSO to 50 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Functional Studies - Baicalein (ab120723)
ab51608 staining HIF1α in HepG2 cells treated with baicalein (ab120723), by ICC/IF. Increase in HIF1α expression correlates with increased concentration of baicalein as described in literature. The cells were incubated at 37°C for 6h in media containing different concentrations of ab120723 (baicalein) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab51608 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
This product has been referenced in:
Lu JH et al. Baicalein inhibits formation of a-synuclein oligomers within living cells and prevents Aß peptide fibrillation and oligomerisation. Chembiochem12:615-24 (2011).
Read more (PubMed: 21271629) »
Deschamps JD et al. Baicalein is a potent in vitro inhibitor against both reticulocyte 15-human and platelet 12-human lipoxygenases. Bioorg Med Chem14:4295-301 (2006).
Read more (PubMed: 16500106) »
Heo HJ et al. Potent Inhibitory effect of flavonoids in Scutellaria baicalensis on amyloid beta protein-induced neurotoxicity. J Agric Food Chem52:4128-32 (2004).
Read more (PubMed: 15212458) »
Lee JH et al. The role of Ca2+ in baicalein-induced apoptosis in human breast MDA-MB-231 cancer cells through mitochondria- and caspase-3-dependent pathway. Anticancer Res28:1701-11 (0).
Read more (PubMed: 18630529) »