Overview

  • Product nameAnti-Bak antibody
    See all Bak primary antibodies
  • Description
    Rabbit polyclonal to Bak
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 23-38 (with C-terminal Lysine added) of Human Bak.

  • Positive control
    • A431 whole cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab10430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: 1/100 (human colon adenocarcinoma).
    WB: 1/2000. Detects a band of approximately 28 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIn the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.
    • Tissue specificityExpressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
    • Sequence similaritiesBelongs to the Bcl-2 family.
    • DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
    • Cellular localizationMitochondrion membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptosis regulator BAK antibody
      • BAK antibody
      • BAK like antibody
      • Bak NT antibody
      • BAK_HUMAN antibody
      • Bak1 antibody
      • Bcl 2 homologous antagonist/killer antibody
      • Bcl 2 like 7 protein antibody
      • Bcl-2 homologous antagonist/killer antibody
      • Bcl-2-like protein 7 antibody
      • BCL2 antagonist/killer 1 antibody
      • Bcl2 like 7 Protein antibody
      • Bcl2-L-7 antibody
      • BCL2L7 antibody
      • CDN1 antibody
      • Cell death inhibitor 1 antibody
      • MGC117255 antibody
      • MGC3887 antibody
      • NBak antibody
      • Pro apoptotic protein BAK antibody
      see all

    References for Anti-Bak antibody (ab10430)

    ab10430 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab10430.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"