The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 (human colon adenocarcinoma).
WB: 1/2000. Detects a band of approximately 28 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.
Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
Belongs to the Bcl-2 family.
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.