Overview

  • Product nameAnti-Bak antibody
    See all Bak primary antibodies
  • Description
    Rabbit polyclonal to Bak
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Hamster, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide derived from the sequence of human Bak, conjugated to KLH.

  • Positive control
    • A431 or HeLa cells. Cervical cancer tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IP 1/100.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionIn the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.
  • Tissue specificityExpressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localizationMitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis regulator BAK antibody
    • BAK antibody
    • BAK like antibody
    • Bak NT antibody
    • BAK_HUMAN antibody
    • Bak1 antibody
    • Bcl 2 homologous antagonist/killer antibody
    • Bcl 2 like 7 protein antibody
    • Bcl-2 homologous antagonist/killer antibody
    • Bcl-2-like protein 7 antibody
    • BCL2 antagonist/killer 1 antibody
    • Bcl2 like 7 Protein antibody
    • Bcl2-L-7 antibody
    • BCL2L7 antibody
    • CDN1 antibody
    • Cell death inhibitor 1 antibody
    • MGC117255 antibody
    • MGC3887 antibody
    • NBak antibody
    • Pro apoptotic protein BAK antibody
    see all

Anti-Bak antibody images

  • Anti-Bak antibody (ab69404) at 1/1000 dilution + A431 cell lysate

    Predicted band size : 23 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent staining of HeLa cells using ab69404. nuclei were counter-stained using DRAQ5.
  • Immunohistochemical analysis of human cervical cancer tissue using 1/50 ab69404.

References for Anti-Bak antibody (ab69404)

ab69404 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Dog Cell lysate - whole cell (CPEK)
Gel Running Conditions Reduced Denaturing (4-20% polyacrylamide)
Loading amount 20 µg
Specification CPEK
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Apr 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"