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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Purity Protein A purified
Clone number AT8B4
Light chain type kappa
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500. Predicted molecular weight: 23 kDa.
1/250 - 1/500.
Function In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.
Tissue specificity Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
Sequence similarities Belongs to the Bcl-2 family.
Domain Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Cellular localization Mitochondrion membrane.
Information by UniProt
Apoptosis regulator BAK antibody
BAK like antibody
Anti-Bak antibody [AT8B4] images
Western blot - Bak antibody [AT8B4] (ab104124)
Anti-Bak antibody [AT8B4] (ab104124) at 1/500 dilution + Mouse lung lyasate at 35 µg Secondary Goat anti-mouse HRP conjugate developed using the ECL technique Predicted band size : 23 kDa
Immunocytochemistry/ Immunofluorescence - Bak antibody [AT8B4] (ab104124)
Immunofluorescence staining of Bak in HeLa cells using Hoechst 3342 (Blue) for nucleus staining and ab104124 antibody (1/500) with Texas Red (Red).
References for Anti-Bak antibody [AT8B4] (ab104124)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"