Anti-BAMBI/NMA antibody (ab203070)
Key features and details
- Rabbit polyclonal to BAMBI/NMA
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-BAMBI/NMA antibody
See all BAMBI/NMA primary antibodies -
Description
Rabbit polyclonal to BAMBI/NMA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig -
Immunogen
Synthetic peptide corresponding to Human BAMBI/NMA conjugated to keyhole limpet haemocyanin.
Database link: Q13145 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X
Aqueous buffered solution -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
When using a fluorescent probe the recommended dilution is 1/50 - 1/200. |
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WB |
1/100 - 1/1000. Predicted molecular weight: 29 kDa.
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Flow Cyt |
1/20 - 1/100.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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Notes |
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IHC-P
Use at an assay dependent concentration. When using a fluorescent probe the recommended dilution is 1/50 - 1/200. |
WB
1/100 - 1/1000. Predicted molecular weight: 29 kDa. |
Flow Cyt
1/20 - 1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Negatively regulates TGF-beta signaling. -
Tissue specificity
High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested. -
Sequence similarities
Belongs to the BAMBI family. -
Developmental stage
Expression in poorly metastatic human melanoma cell lines; no expression in highly metastatic human melanoma cell lines. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 25805 Human
- Entrez Gene: 68010 Mouse
- Entrez Gene: 449001 Pig
- Entrez Gene: 83837 Rat
- Omim: 604444 Human
- SwissProt: Q13145 Human
- SwissProt: Q9D0L6 Mouse
- SwissProt: Q91XN4 Rat
see all -
Alternative names
- BAMBI antibody
- BAMBI_HUMAN antibody
- BMP and activin membrane bound inhibitor homolog antibody
see all
Images
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All lanes : Anti-BAMBI/NMA antibody (ab203070) at 1/200 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat kidney tissue lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 29 kDa -
Flow cytometric analysis of Mouse splenocytes labeling BAMBI/NMA with ab203070 at 1/100 dilution (green) followed by a FITC conjugated secondary, compared with negative control cells (blue).
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab203070 has been referenced in 9 publications.
- Hoang NTD et al. BMP and activin membrane-bound inhibitor regulate connective tissue growth factor controlling mesothelioma cell proliferation. BMC Cancer 22:984 (2022). PubMed: 36109807
- Pan Z et al. LncRNA prostate androgen-regulated transcript 1 (PART 1) functions as an oncogene in osteosarcoma via sponging miR-20b-5p to upregulate BAMBI. Ann Transl Med 9:488 (2021). PubMed: 33850885
- Liu W et al. MSC-derived small extracellular vesicles overexpressing miR-20a promoted the osteointegration of porous titanium alloy by enhancing osteogenesis via targeting BAMBI. Stem Cell Res Ther 12:348 (2021). PubMed: 34134765
- Zhaosong M et al. Heterogeneity affects the differentiation potential of dental follicle stem cells through the TGF-β signaling pathway. Bioengineered 12:12294-12307 (2021). PubMed: 34927533
- Chen H et al. Regeneration of pulpo-dentinal-like complex by a group of unique multipotent CD24a+ stem cells. Sci Adv 6:eaay1514 (2020). PubMed: 32284993
- Wang Z et al. Long Non-Coding RNA PVT1 Regulates BAMBI To Promote Tumor Progression In Non-Small Cell Lung Cancer By Sponging miR-17-5p. Onco Targets Ther 13:131-142 (2020). PubMed: 32021261
- Chen W et al. Kangquan Recipe Regulates the Expression of BAMBI Protein via the TGF-ß/Smad Signaling Pathway to Inhibit Benign Prostatic Hyperplasia in Rats. Evid Based Complement Alternat Med 2019:6281819 (2019). PubMed: 31186664
- Yang Y et al. BAMBI inhibits inflammation through the activation of autophagy in experimental spinal cord injury. Int J Mol Med 39:423-429 (2017). PubMed: 28035406
- Wang X et al. BAMBI overexpression together with ß-sitosterol ameliorates NSCLC via inhibiting autophagy and inactivating TGF-ß/Smad2/3 pathway. Oncol Rep 37:3046-3054 (2017). PubMed: 28440452