Overview

  • Product nameAnti-BAMBI antibody
    See all BAMBI primary antibodies
  • Description
    Mouse monoclonal to BAMBI
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-261 of Human BAMBI

Properties

Applications

Our Abpromise guarantee covers the use of ab57043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 29 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionNegatively regulates TGF-beta signaling.
  • Tissue specificityHigh expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested.
  • Sequence similaritiesBelongs to the BAMBI family.
  • Developmental stageExpression in poorly metastatic human melanoma cell lines; no expression in highly metastatic human melanoma cell lines.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAMBI antibody
    • BAMBI_HUMAN antibody
    • BMP and activin membrane bound inhibitor homolog antibody
    • BMP and activin membrane-bound inhibitor homolog antibody
    • NMA antibody
    • Non metastatic gene A protein antibody
    • Non-metastatic gene A protein antibody
    • Putative transmembrane protein NMA antibody
    see all

Anti-BAMBI antibody images



  • Predicted band size : 29 kDa
    BAMBI antibody (ab57043) at 1ug/lane + K-562 cell lysate at 25ug/lane.
  • Overlay histogram showing K562 cells stained with ab57043 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57043, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-BAMBI antibody (ab57043)

This product has been referenced in:
  • Wanninger J  et al. Adiponectin induces the transforming growth factor decoy receptor BAMBI in human hepatocytes. FEBS Lett 585:1338-44 (2011). WB ; Human . Read more (PubMed: 21496456) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 0,3% Tritòn X-100
Sample Rat Cell (adult Cardiac Fibroblast)
Specification adult Cardiac Fibroblast
Gating Strategy 10000 cells
Preparation Cell harvesting/tissue preparation method: Trip-EDTA
Sample buffer: PBS-BSA
Username

Abcam user community

Verified customer

Submitted Aug 01 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (retinal ganglion cells)
Specification retinal ganglion cells
Permeabilization Yes - triton X
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

Submitted May 22 2014




I am very pleased to hear you would like to accept our offer and test ab57043 Anti-BAMBI antibody in mouse. This code will give you: 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for ...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung tissue)
Specification Lung tissue
Fixative HOPE
Antigen retrieval step None
Permeabilization No
Blocking step Zytomed Systems Blocking Solution as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 02 2011

Application Western blot
Sample Human Cell lysate - whole cell (A549 Lung epithelial cell line/ lung cancer cell l)
Loading amount 250000 cells
Specification A549 Lung epithelial cell line/ lung cancer cell l
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"