Anti-Band 3 (phospho Y359) antibody [EPR1427Y] (ab77236)


  • Product nameAnti-Band 3 (phospho Y359) antibody [EPR1427Y]
    See all Band 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR1427Y] to Band 3 (phospho Y359)
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding tyrosine 359 of human Band 3.

  • Positive control
    • Red blood cell lysates treated with pervanadate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab77236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Predicted molecular weight: 102 kDa.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionFunctions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
    • Tissue specificityDetected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
    • Involvement in diseaseOvalocytosis, Southeast Asian
      Spherocytosis 4
      Renal tubular acidosis, distal, autosomal dominant
      Renal tubular acidosis, distal, with hemolytic anemia
      Renal tubular acidosis, distal, with normal red cell morphology
    • Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
    • Post-translational
      Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
      Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
    • Cellular localizationCell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
    • Information by UniProt
    • Database links
    • Alternative names
      • AE 1 antibody
      • AE1 antibody
      • Anion exchange protein 1 antibody
      • Anion exchanger 1 antibody
      • B3AT_HUMAN antibody
      • Band 3 anion transport protein antibody
      • BND3 antibody
      • CD233 antibody
      • DI antibody
      • Diego blood group antibody
      • EMPB3 antibody
      • EPB3 antibody
      • Erythrocyte membrane protein band 3 antibody
      • Erythroid anion exchange protein antibody
      • FR antibody
      • Froese blood group antibody
      • RTA1A antibody
      • SLC4A1 antibody
      • Solute carrier family 4 anion exchanger member 1 antibody
      • Solute carrier family 4 member 1 antibody
      • SW antibody
      • Swann blood group antibody
      • Waldner blood group antibody
      • WD antibody
      • WD1 antibody
      • WR antibody
      • Wright blood group antibody
      see all

    Anti-Band 3 (phospho Y359) antibody [EPR1427Y] images

    • All lanes : Anti-Band 3 (phospho Y359) antibody [EPR1427Y] (ab77236) at 1/50000 dilution

      Lane 1 : Red blood cell lysate, untreated.
      Lane 2 : Red blood cell lysate treated with pervanadate.

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

    References for Anti-Band 3 (phospho Y359) antibody [EPR1427Y] (ab77236)

    ab77236 has not yet been referenced specifically in any publications.

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    We currently do not have Rhesus Monkey Band 3 specific antibodies. The Band 3 antibodies we have are ab11012, ab77236, ab78067, ab104998, ab108414, ab125070 and they seems specific to N-terminal cytopl...

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