Overview

  • Product nameAnti-BANF1 antibody
    See all BANF1 primary antibodies
  • Description
    Rabbit polyclonal to BANF1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide containing 15 amino acids from near the C terminus of BANF1 (Human) conjugated to KLH

  • Positive control
    • Human heart tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 10 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionPlays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
  • Tissue specificityWidely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the BAF family.
  • DomainHas a helix-hairpin-helix (HhH) structural motif conserved among proteins that bind non-specifically to DNA.
    LEM domain proteins bind centrally on the BAF dimer, whereas DNA binds to the left and right sides.
  • Post-translational
    modifications
    Partially phosphorylated on serine. Ser-4 phosphorylation may block BAF ability to promote EMD binding to lamins in vitro. Non phosphorylated BAF seems to enhances binding between EMD and LMNA.
  • Cellular localizationNucleus. Cytoplasm. Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAF antibody
    • BAF_HUMAN antibody
    • BANF 1 antibody
    • BANF1 antibody
    • Barrier to autointegration factor 1 antibody
    • Barrier to autointegration factor antibody
    • Barrier-to-autointegration factor antibody
    • BCRG 1 antibody
    • BCRG1 antibody
    • BCRP 1 antibody
    • BCRP1 antibody
    • Breakpoint cluster region protein 1 antibody
    • D14S1460 antibody
    • MGC111161 antibody
    • NGPS antibody
    see all

Anti-BANF1 antibody images

  • Human heart (formalin fixed, paraffin embedded) stained with ab115226 at 5 ug/ml followed by biotinylated Goat anti Rabbit IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.

References for Anti-BANF1 antibody (ab115226)

ab115226 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"