• Product name
  • Description
    Mouse monoclonal to BANF1
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length BANF1 protein (Human) aas 1-90, with a 26 kDa tag, AAH05942.

  • Positive control
    • Recombinant protein (immunogen), Jurkat cell lysate and HeLa cells.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab129184 as a replacement.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab88464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 10 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt 1/50. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • Function
    Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
  • Tissue specificity
    Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the BAF family.
  • Domain
    Has a helix-hairpin-helix (HhH) structural motif conserved among proteins that bind non-specifically to DNA.
    LEM domain proteins bind centrally on the BAF dimer, whereas DNA binds to the left and right sides.
  • Post-translational
    Partially phosphorylated on serine. Ser-4 phosphorylation may block BAF ability to promote EMD binding to lamins in vitro. Non phosphorylated BAF seems to enhances binding between EMD and LMNA.
  • Cellular localization
    Nucleus. Cytoplasm. Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAF antibody
    • BAF_HUMAN antibody
    • BANF 1 antibody
    • BANF1 antibody
    • Barrier to autointegration factor 1 antibody
    • Barrier to autointegration factor antibody
    • Barrier-to-autointegration factor antibody
    • BCRG 1 antibody
    • BCRG1 antibody
    • BCRP 1 antibody
    • BCRP1 antibody
    • Breakpoint cluster region protein 1 antibody
    • D14S1460 antibody
    • MGC111161 antibody
    • NGPS antibody
    see all


  • Anti-BANF1 antibody (ab88464) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µg

    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 10 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Anti-BANF1 antibody (ab88464) at 5 µg/ml + Jurkat cell lysate at 25 µg

    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 10 kDa
    Observed band size : 8 kDa (why is the actual band size different from the predicted?)
  • ab88464 at 10µg/ml staining BANF1 in Hela cells.
  • Overlay histogram showing HepG2 cells stained with ab88464 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88464, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Eisch V  et al. Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts. Oncotarget 7:24700-18 (2016). Read more (PubMed: 27015553) »
  • Paquet N  et al. Néstor-Guillermo Progeria Syndrome: a biochemical insight into Barrier-to-Autointegration Factor 1, alanine 12 threonine mutation. BMC Mol Biol 15:27 (2014). WB . Read more (PubMed: 25495845) »

See all 3 Publications for this product

Customer reviews and Q&As

Western blot
Mouse Cell lysate - whole cell (C2C12 myoblast)
Loading amount
10 µg
C2C12 myoblast
Gel Running Conditions
Reduced Denaturing (16%)
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 22 2012

Thank you for your participation in Abcam's follow up survey.

Please let me know how the free of charge replacement vial worked for you - once you have tried it.

If there is anything else I can do for you, or if you have questions t...

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We do not have any information about the new lot, other than that it has passed quality control. We have not received any complaints about it. The new lot is ****. If you do place another order for ab88464, you will want to specify that you want this p...

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Thank you for sending your protocol details. The one suggestion I have that may improve the signal strength is to switch the membrane blocking buffer from 5% milk to 1% BSA. The antigen recognition of different batches of polyclonal antibodies is often...

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Thank you for bringing this to our attention. I am sorry to read that you are having difficulty with these vials. Please send a few details about your samples (tissue type and species and how the samples were prepared), and the protocol. We would like ...

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