Overview

  • Product nameAnti-BANF2 antibody
    See all BANF2 primary antibodies
  • Description
    Rabbit polyclonal to BANF2
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: FLSEPIGEKD VCWVDGISHE LAINLVTKGI NKAYILLGQF LLMHKNEAEF QRWLICCFGA TECEAQQTSH CLKEWCA, corresponding to amino acids 11-87 of Human BANF2.

  • Positive control
    • Human pancreas tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab122806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Target

  • FunctionMay play a role in BANF1 regulation and influence tissue-specific roles of BANF1.
  • Tissue specificityExpressed strongly in testis and pancreas. Also detected in brain, colon, liver, lung, ovary, placenta, prostate, small intestine, spleen and thymus. Not detected in heart, kidney and skeletal muscle.
  • Sequence similaritiesBelongs to the BAF family.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAF-L antibody
    • BAF2 antibody
    • BAFL antibody
    • BAFL_HUMAN antibody
    • BANF2 antibody
    • Barrier-to-autointegration factor 2 antibody
    • Barrier-to-autointegration factor-like protein antibody
    • C20orf179 antibody
    see all

Anti-BANF2 antibody images

  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
  • ab122806, at 1/50 dilution, staining BANF2 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli using ab122806.
    Recommended treatment:
    Fixation/Permeabilization: PFA/Triton X-100

References for Anti-BANF2 antibody (ab122806)

ab122806 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"