Overview

  • Product name
    Anti-BANF2 antibody
  • Description
    Rabbit polyclonal to BANF2
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: FLSEPIGEKD VCWVDGISHE LAINLVTKGI NKAYILLGQF LLMHKNEAEF QRWLICCFGA TECEAQQTSH CLKEWCA, corresponding to amino acids 11-87 of Human BANF2.

  • Positive control
    • Human pancreas tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Target

  • Function
    May play a role in BANF1 regulation and influence tissue-specific roles of BANF1.
  • Tissue specificity
    Expressed strongly in testis and pancreas. Also detected in brain, colon, liver, lung, ovary, placenta, prostate, small intestine, spleen and thymus. Not detected in heart, kidney and skeletal muscle.
  • Sequence similarities
    Belongs to the BAF family.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAF-L antibody
    • BAF2 antibody
    • BAFL antibody
    • BAFL_HUMAN antibody
    • BANF2 antibody
    • Barrier-to-autointegration factor 2 antibody
    • Barrier-to-autointegration factor-like protein antibody
    • C20orf179 antibody
    see all

Anti-BANF2 antibody images

  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
  • ab122806, at 1/50 dilution, staining BANF2 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli using ab122806.
    Recommended treatment:
    Fixation/Permeabilization: PFA/Triton X-100

References for Anti-BANF2 antibody (ab122806)

ab122806 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122806.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up