Overview

  • Product nameAnti-BAP31 antibody [7A3BB6]
    See all BAP31 primary antibodies
  • Description
    Mouse monoclonal [7A3BB6] to BAP31
  • SpecificityShotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WB, IP, In-Cell ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Hela cell fraction

  • Positive control
    • HepG2 cell lysate; HL60 and Human fibroblast cells. Human normal colon FFPE tissue.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 99.98% HBS
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesab112993 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE.
  • ClonalityMonoclonal
  • Clone number7A3BB6
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 28 kDa.
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseNote=BCAP31 is deleted in the chromosome Xq28 deletion syndrome which involves BCAP31 and the and the promoter region of ABCD1.
  • Sequence similaritiesBelongs to the BCAP29/BCAP31 family.
  • Post-translational
    modifications
    Cleaved by CASP8 and other caspases.
  • Cellular localizationEndoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6C6 AG antibody
    • 6C6 AG tumor associated antigen antibody
    • 6C6-AG tumor-associated antigen antibody
    • 6C6AG antibody
    • 6C6AG tumor associated antigen antibody
    • Accessory protein BAP 31 antibody
    • Accessory protein BAP31 antibody
    • B cell receptor associated protein 31 antibody
    • B-cell receptor-associated protein 31 antibody
    • BA31 antibody
    • BAP 31 antibody
    • Bap31 antibody
    • BAP31_HUMAN antibody
    • BCAP 31 antibody
    • BCAP31 antibody
    • BCR associated protein Bap 31 antibody
    • BCR associated protein Bap31 antibody
    • BCR-associated protein 31 antibody
    • CDM antibody
    • CDM protein antibody
    • DXS1357E antibody
    • MS950 antibody
    • p28 antibody
    • p28 Bap31 antibody
    • Protein CDM antibody
    • RP23-329M9.5 antibody
    see all

Anti-BAP31 antibody [7A3BB6] images

  • All lanes : Anti-BAP31 antibody [7A3BB6] (ab112993) at 0.5 µg/ml

    Lane 1 : HepG2 Cell Lysate
    Lane 2 : H9C2 Cell Lysate
    Lane 3 : MEF Cell Lysate


    Predicted band size : 28 kDa
  • ab112993 at 1ug/ml staining BAP31 in MeOH-fixed HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).
  • ab112993 at 5 µg/ml staining BAP31 in Human fibroblast cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly in the endoplasmic reticulum.
  • IHC image of BAP31 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112993, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-BAP31 antibody [7A3BB6] (ab112993)

ab112993 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"