Biological descriptionSelective Ca2+ chelator. Useful for measurement and manipulation of intracellular free Ca2+ levels. Also displays potent actin and microtubule depolymerizing activity independent of Ca2+ chelation.
Chemical name1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid disodium salt
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 50 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
BAPTA sodium salt images
Functional Studies-BAPTA sodium salt(ab120449)
ab66705 staining PAI1 in HepG2 cells treated with BAPTA sodium salt (ab120449), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of BAPTA sodium salt, as described in literature. The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120449 (BAPTA sodium salt) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
References for BAPTA sodium salt (ab120449)
This product has been referenced in:
Hong JH et al. Intracellular calcium spikes in rat suprachiasmatic nucleus neurons induced by BAPTA-based calcium dyes. PLoS One5:e9634 (2010).
Read more (PubMed: 20224788) »