Overview

  • Product nameAnti-BAPX1 antibody
    See all BAPX1 primary antibodies
  • Description
    Rabbit polyclonal to BAPX1
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (GAGGGGGSGP AGVAEEEEEP AAPKPRKKRS RAAFSHAQVF ELERRFNHQR) of human BAPX1 (NP_001180).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

Target

  • FunctionTranscriptional repressor that acts as a negative regulator of chondrocyte maturation. PLays a role in distal stomach development; required for proper antral-pyloric morphogenesis and development of antral-type epithelium. In concert with GSC, defines the structural components of the middle ear; required for tympanic ring and gonium development and in the regulation of the width of the malleus.
  • Tissue specificityExpressed at highest levels in cartilage, bone (osteosarcoma) and gut (small intestine and colon), whereas moderate expression is seen in trachea and brain. Expressed in visceral mesoderm and embryonic skeleton.
  • Involvement in diseaseDefects in NKX3-2 are the cause of spondylo-megaepiphyseal-metaphyseal dysplasia (SMMD) [MIM:613330]. It is a skeletal dysplasia characterized by disproportionate short stature with a short and stiff neck and trunk, relatively long limbs that may show flexion contractures of the distal joints, delayed and impaired ossification of the vertebral bodies, the presence of large epiphysea ossification centers and wide growth plates in the long tubular bones and numerous pseudoepiphyses of the short tubular bones in hands and feet.
  • Sequence similaritiesBelongs to the NK-3 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bagpipe homeobox 1 antibody
    • Bagpipe homeobox homolog 1 antibody
    • Bagpipe homeobox protein homolog 1 antibody
    • Bagpipe homeobox, Drosophila, homolog of, 1 antibody
    • BAPX 1 antibody
    • Homeobox protein NK-3 homolog B antibody
    • Homeobox protein Nkx 3.2 antibody
    • Homeobox protein Nkx-3.2 antibody
    • MGC138171 antibody
    • NK3 homeobox 2 antibody
    • NKX3 2 antibody
    • NKX3-2 antibody
    • NKX3.2 antibody
    • NKX3.2, mouse, homolog of antibody
    • NKX32_HUMAN antibody
    • NKX3B antibody
    • OTTHUMP00000115816 antibody
    • SMMD antibody
    see all

Anti-BAPX1 antibody images

  • Anti-BAPX1 antibody (ab83288) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab83288 stained Hek293 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83288, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-BAPX1 antibody (ab83288)

This product has been referenced in:
  • Kamel G  et al. Requirement for frzb and fzd7a in cranial neural crest convergence and extension mechanisms during zebrafish palate and jaw morphogenesis. Dev Biol 381:423-33 (2013). Read more (PubMed: 23806211) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - nuclear (Mouse limb bud micro-mass culture)
Specification Mouse limb bud micro-mass culture
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Min-Su Han

Verified customer

Submitted May 09 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"