Overview

  • Product nameAnti-BARD1 antibody [2059C4a]
    See all BARD1 primary antibodies
  • Description
    Mouse monoclonal [2059C4a] to BARD1
  • Tested applicationsSuitable for: Dot blot, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control
    • HEK293 whole cell lysate, RIPA lysate of HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab50984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
WB 1/50. Predicted molecular weight: 86 kDa.
IP 1/50.

Target

  • FunctionProbable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 4 ANK repeats.
    Contains 2 BRCT domains.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.
  • Cellular localizationNucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • BARD-1 antibody
    • Bard1 antibody
    • BARD1_HUMAN antibody
    • BRCA1 associated RING domain 1 antibody
    • BRCA1 associated RING domain gene 1 antibody
    • BRCA1 associated RING domain protein 1 antibody
    • BRCA1-associated RING domain protein 1 antibody
    see all

Anti-BARD1 antibody [2059C4a] images

  • Anti-BARD1 antibody [2059C4a] (ab50984) at 1/50 dilution + HEK293 whole cell lysate at 50 µg

    Secondary
    Anti mouse IgG antibody at 1/2500 dilution

    Predicted band size : 86 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Ab50984 at 1/50 dilution precipitating RIPA lysate of HeLa cells at 500µg; visualised with anti mouse IgG antibody at 1/2500 diltuion.

References for Anti-BARD1 antibody [2059C4a] (ab50984)

This product has been referenced in:
  • Stecklein SR  et al. BRCA1 and HSP90 cooperate in homologous and non-homologous DNA double-strand-break repair and G2/M checkpoint activation. Proc Natl Acad Sci U S A 109:13650-5 (2012). WB . Read more (PubMed: 22869732) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"