Overview

  • Product nameAnti-BARD1 antibody
    See all BARD1 primary antibodies
  • Description
    Rabbit polyclonal to BARD1
  • Tested applicationsSuitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide:

    CSKLRNLLHDNELSDLK

    conjugated to KLH, corresponding to amino acids 108-124 of Human BARD1.

  • Positive control
    • Human skeletal muscle and skin tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab115477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1 - 1/1000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionProbable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 4 ANK repeats.
    Contains 2 BRCT domains.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.
  • Cellular localizationNucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • BARD-1 antibody
    • Bard1 antibody
    • BARD1_HUMAN antibody
    • BRCA1 associated RING domain 1 antibody
    • BRCA1 associated RING domain gene 1 antibody
    • BRCA1 associated RING domain protein 1 antibody
    • BRCA1-associated RING domain protein 1 antibody
    see all

Anti-BARD1 antibody images

  • ab115477, at 10µg/ml, staining BARD1 in formalin-fixed, paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry, using a biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115477, at 10µg/ml, staining BARD1 in formalin-fixed, paraffin-embedded Human skin tissue by Immunohistochemistry, using a biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-BARD1 antibody (ab115477)

ab115477 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (EC109)
Gel Running Conditions Non-reduced Denaturing (12% SDS)
Loading amount 80 µg
Treatment CHX for indicated time points
Specification EC109
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Dec 31 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"