Overview

  • Product nameAnti-BARD1 antibody
    See all BARD1 primary antibodies
  • Description
    Rabbit polyclonal to BARD1
  • Specificityab69645 detects endogenous levels of total BARD1 protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from N-terminal of human BARD1.

  • Positive control
    • HeLa cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab69645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).

Target

  • FunctionProbable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 4 ANK repeats.
    Contains 2 BRCT domains.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.
  • Cellular localizationNucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • BARD-1 antibody
    • Bard1 antibody
    • BARD1_HUMAN antibody
    • BRCA1 associated RING domain 1 antibody
    • BRCA1 associated RING domain gene 1 antibody
    • BRCA1 associated RING domain protein 1 antibody
    • BRCA1-associated RING domain protein 1 antibody
    see all

Anti-BARD1 antibody images

  • All lanes : Anti-BARD1 antibody (ab69645) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 86 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-BARD1 antibody (ab69645)

ab69645 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"