Overview

  • Product nameAnti-BarH2 antibody
  • Description
    Rabbit polyclonal to BarH2
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: REITSSRESP PVRAKKPRKA RTAFSDHQLN QLERSFERQK YLSVQDRMDL, corresponding to a region within internal sequence amino acids 216-265 of human BarH2 (NP_064447).

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab82639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceThe Drosophila BarH1 and BarH2 (Bar) genes are homeobox containing genes, which are required for the fate determination of external sensory organs in the fly. Using a bioinformatic approach, murine and human homeobox genes highly related to the Bar Drosophila genes were identified, which were designated Barhl1 and Barhl2. Murine Barhl2 is the homolog of the rat mBH1 gene. The human BARHL2 fragment shares 92% and 74% nucleotide identity, respectively, with the rat mBH1 and human BARHL1 cDNA sequences.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • BARH LIKE 2 antibody
    • BarH like 2 homeobox protein antibody
    • BARHL2 antibody

Anti-BarH2 antibody images

  • Anti-BarH2 antibody (ab82639) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

References for Anti-BarH2 antibody (ab82639)

ab82639 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"