Anti-BarX1 antibody (ab26156)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab26156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceBarX1 belongs to the Bar subclass of the homeobox gene family. The function of this gene has not yet been determined; however, studies in the mouse and chick homolog suggest a role in developing teeth and craniofacial mesenchyme of neural crest origin. The role of these homologs implicates the human gene as a candidate for unmapped disorders involving tooth and jaw development.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • BarH like homeobox 1 antibody
    • BarX 1 antibody
    • Homeobox protein BarH like 1 antibody

Anti-BarX1 antibody images

  • Anti-BarX1 antibody (ab26156) at 0.5 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50000 - 1/100000

    Predicted band size : 24 kDa
  • Paraffin embeded Tissue: Human Heart
    Cells with positive label: Myocardial cells (indicated with arrows)
    Antibody concentration:4-8µg/ml
    Magnification:400X
  • Paraffin embeded Tissue: Human Pancreas
    Cells with positive label: Epithelial cells of pancreatic acinus (indicated with arrows)
    Antibody concentration:4-8µg/ml
    Magnification:400X

References for Anti-BarX1 antibody (ab26156)

ab26156 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tooth)
Antigen retrieval step None
Permeabilization No
Specification tooth
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 19 2015

Thank you for your reply. It is unfortunate that the sequence information for pig is not available. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on...

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Thank you for your enquiry. To our knowledge, the antibody has yet to be tested in pig. All tested species are listed on Abcam product datasheets. I am, however, enquiring with the originator of this product to find out the homology between the imm...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (mandible, digestive tract, stomach)
Loading amount 30 µg
Specification mandible, digestive tract, stomach
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Isabelle Miletich

Verified customer

Submitted Oct 25 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"