Anti-Basal Bodies of Cillia antibody [LhS28] (ab14373)

Overview

  • Product nameAnti-Basal Bodies of Cillia antibody [LhS28]
  • Description
    Mouse monoclonal [LhS28] to Basal Bodies of Cillia
  • SpecificityThis antibody recognises the basal bodies of cillia in all cilliated cells.
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Cytoskeletal preparation of the BHK a21 cell line expressing cillia basal bodies (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab14373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceFlagella and cillia both project from the cell surface and beat in distinctive patterns. Flagella are whiplike tails that propel many free-living cells, such as sperm, through fluid environments. Cilia are shorter and usually more profuse than flagella. In both flagella and cilia, nine pairs of microtubules ring two central microtubules. This arrangement is called a "9 + 2 array." A system of spokes and links holds the arrangement together. Microtubules of a flagellum or cilium arise from centrioles, which remain at the base of the completed structure as a basal body. These are sites of dense material that generate large numbers of microtubules.

References for Anti-Basal Bodies of Cillia antibody [LhS28] (ab14373)

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Bos taurus Tissue sections (Oviduct, ciliated cells)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Permeabilization No
Specification Oviduct, ciliated cells
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 08 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"