Anti-basal cell Cytokeratin antibody [RCK103] (ab9222)

Overview

  • Product nameAnti-basal cell Cytokeratin antibody [RCK103]
  • Description
    Mouse monoclonal [RCK103] to basal cell Cytokeratin
  • SpecificityThis antibody recognizes (amongst others) cytokeratin 5. It stains basal cells in combined and stratified epithelial tissues. It also recognizes the stem cell population, including the so-called amplifying cells in the prostate epithelium.
  • Tested applicationsSuitable for: Dot blot, Flow Cyt, IHC-Fr, ICC, WBmore details
  • Species reactivity
    Reacts with: Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Dog, Human, Pig, Quail
    Does not react with: Zebrafish
  • Immunogen

    Mix of cell preparations containing human cytokeratins.

  • General notes


    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
  • ClonalityMonoclonal
  • Clone numberRCK103
  • MyelomaSp2/0-Ag14
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/100 - 1/1000.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/100 - 1/200.
ICC Use at an assay dependent concentration.
WB 1/100 - 1/1000.

Anti-basal cell Cytokeratin antibody [RCK103] images

  • Immunohistochemistry on frozen sections of human skin
  • Overlay histogram showing A431 cells stained with ab9222 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9222, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunofluorescence staining of a 7 days old zebrafish embryo, showing positive reactivity in epithelia lining the intestine and internal organs

References for Anti-basal cell Cytokeratin antibody [RCK103] (ab9222)

This product has been referenced in:
  • Kuzinski J  et al. Modulation of vH+-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet. Am J Physiol Regul Integr Comp Physiol 303:R909-20 (2012). Flow Cyt ; Sheep . Read more (PubMed: 22972839) »
  • Halkidou K  et al. Upregulation and nuclear recruitment of HDAC1 in hormone refractory prostate cancer. Prostate 59:177-89 (2004). Read more (PubMed: 15042618) »

See all 3 Publications for this product

Product Wall

Thank you for your reply. I just checked our catalogue and unfortunately we do not have any positive control recombinant cytokeratin 5 proteins. This monoclonal antibody stains basal cells in combined and stratified epithelial tissues. It recog...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"