Overview

  • Product nameAnti-BASC4 antibody
    See all BASC4 primary antibodies
  • Description
    Rabbit polyclonal to BASC4
  • Specificityab75000 detects endogenous levels of total BASC4 protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal region of the human protein.

  • Positive control
    • IHC-P: Human tonsil tissue WB: K562 cell extract

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • Tissue specificityBrain, thymus, spleen, kidney and placenta. Overexpressed in most breast cancer cell lines.
  • Involvement in diseaseNote=A chromosomal aberration involving BCAS4 has been found in some breast carcinoma cell lines. Translocation t(17;20)(q23;q13) with BCAS3.
  • Sequence similaritiesBelongs to the cappuccino family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCAS4 antibody
    • BCAS4/BCAS3 fusion antibody
    • BCAS4_HUMAN antibody
    • Breast carcinoma amplified sequence 4 antibody
    • Breast carcinoma amplified sequence 4/3 fusion protein antibody
    • Breast carcinoma-amplified sequence 4 antibody
    • CNOL antibody
    • FLJ20495 antibody
    • RP5-1071L10.2 antibody
    see all

Anti-BASC4 antibody images

  • Immunohistochemical analysis of paraffin embedded human tonsil tissue using ab75000 at 1/50 dilution. Right panel was also treated with immunising peptide.
  • All lanes : Anti-BASC4 antibody (ab75000) at 1/500 dilution

    Lane 1 : K562 cell extract
    Lane 2 : K562 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 117 kDa,70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-BASC4 antibody (ab75000)

ab75000 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"