Overview

  • Product nameAnti-Bassoon antibody
    See all Bassoon primary antibodies
  • Description
    Rabbit polyclonal to Bassoon
  • SpecificityNo cross-reactivity to Piccolo. Multiple isoforms can be detected.
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein with His tag, corresponding to amino acids 786-1041 of Human Bassoon.

  • Positive control
    • Mouse and Rat brain lysates; Neuromuscular junction whole muscle tissue from adult mouse.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 416 kDa. For large isoforms reduce dilution.

Target

  • FunctionIs thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act through binding to ERC2/CAST1. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses.
  • Tissue specificityExclusively expressed in brain.
  • Post-translational
    modifications
    Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization.
  • Cellular localizationCytoplasm. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to the active zone of presynaptic density.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bassoon protein antibody
    • bsn antibody
    • BSN_HUMAN antibody
    • KIAA0434 antibody
    • Neuronal double zinc finger protein antibody
    • Presynaptic cytomatrix protein antibody
    • Protein bassoon antibody
    • Zinc finger protein 231 antibody
    • ZNF 231 antibody
    • ZNF231 antibody
    see all

Anti-Bassoon antibody images

  • All lanes : Anti-Bassoon antibody (ab110426) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 416 kDa
  • Immunofluorescence of Neuromuscular junction whole muscle samples from adult mouse, stained with ab110426 at 1/400 dilution. Secondary (Alexa 488 goat antimouse IgG, IgM (H+L). Note: bright staining of the NMJ very selectively and in some terminals, very brightly.

References for Anti-Bassoon antibody (ab110426)

ab110426 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

We have 4 antibodies in our catalog raised against Bassoon: ab82958 (mouse), ab76065 (rabbit), ab110426 (rabbit) and ab101772 (goat).

Could you please let me know what kind of IHC you are planning to pe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"