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Recombinant rat Bassoon protein
Our Abpromise guarantee covers the use of ab82958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 416 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
IHC-P image of Bassoon staining on mouse cerebellum sections using ab82958 (1:1500). The sections were deparaffinized and subjected to heat mediated antigen retrieval using HEIR. The sections were blocked using 1% BSA for 10 mins at 21°C. ab82958 was diluted 1:1500 and incubated with the sctions for 2 hours at 21°C. The secondary antibody used was goat polyclonal to mouse IgG conjugated to Biotin (1:200).
IHC-P image of Bassoon staining on rat cerebellum sections using ab82958 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab82958 was diluted 1:1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to mouse IgG conjugated to biotin (1:200).
IHC image of Bassoon staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82958, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.