Overview

  • Product name
    Anti-Bassoon antibody [SAP7F407]
    See all Bassoon primary antibodies
  • Description
    Mouse monoclonal [SAP7F407] to Bassoon
  • Tested applications
    Suitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant rat Bassoon protein

  • Positive control
    • Rat brain tissue extract This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    SAP7F407
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 416 kDa.
IP Use at an assay dependent concentration.
ICC/IF 1/400.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Is thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act through binding to ERC2/CAST1. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses.
  • Tissue specificity
    Exclusively expressed in brain.
  • Post-translational
    modifications
    Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization.
  • Cellular localization
    Cytoplasm. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to the active zone of presynaptic density.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bassoon protein antibody
    • bsn antibody
    • BSN_HUMAN antibody
    • KIAA0434 antibody
    • Neuronal double zinc finger protein antibody
    • Presynaptic cytomatrix protein antibody
    • Protein bassoon antibody
    • Zinc finger protein 231 antibody
    • ZNF 231 antibody
    • ZNF231 antibody
    see all

Anti-Bassoon antibody [SAP7F407] images

  • IHC-P image of Bassoon staining on mouse cerebellum sections using ab82958 (1:1500). The sections were deparaffinized and subjected to heat mediated antigen retrieval using HEIR. The sections were blocked using 1% BSA for 10 mins at 21°C. ab82958 was diluted 1:1500 and incubated with the sctions for 2 hours at 21°C. The secondary antibody used was goat polyclonal to mouse IgG conjugated to Biotin (1:200).

  • Anti-Bassoon antibody [SAP7F407] (ab82958) at 1/500 dilution + rat brain tissue extract
    Developed using the ECL technique

    Predicted band size : 416 kDa
  • Bassoon localization in the rat brain cerebellar molecular layer using Bassoon monoclonal antibody (ab82958).
  • IHC-P image of Bassoon staining on rat cerebellum sections using ab82958 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab82958 was diluted 1:1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to mouse IgG conjugated to biotin (1:200).

  • IHC image of Bassoon staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82958, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Bassoon antibody [SAP7F407] (ab82958)

This product has been referenced in:
  • Ohn TL  et al. Hair cells use active zones with different voltage dependence of Ca2+ influx to decompose sounds into complementary neural codes. Proc Natl Acad Sci U S A 113:E4716-25 (2016). IHC - Wholemount ; Mouse . Read more (PubMed: 27462107) »
  • Vilmont V  et al. A system for studying mechanisms of neuromuscular junction development and maintenance. Development 143:2464-77 (2016). Read more (PubMed: 27226316) »

See all 12 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Retina Section)
Permeabilization
Yes - Triton X-100
Specification
Retina Section
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 22 2017

Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 24 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Zebra finch Tissue sections (Area X (striatum) brain tissue)
Antigen retrieval step
None
Permeabilization
Yes - PBST
Specification
Area X (striatum) brain tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Mr. Jonnathan Singh

Verified customer

Submitted May 19 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Sample
Rat Cell (hippocampal neuron)
Specification
hippocampal neuron
Permeabilization
Yes - 0.2% TritonX
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 31 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Marmoset (common) Tissue sections (Cerebellum)
Specification
Cerebellum
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 04 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Goat Tissue sections (Cerebellum)
Specification
Cerebellum
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 04 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Cerebellum)
Specification
Cerebellum
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 27 2013

Thank you for contacting us.

This antibody was raised against recombinant rat Bassoon protein and it has not been epitope mapped. It will have one specific binding epitope, however that region has not been determined.

I am sorry t...

Read More

Thank you for contacting us.

We have 4 antibodies in our catalog raised against Bassoon: ab82958 (mouse), ab76065 (rabbit), ab110426 (rabbit) and ab101772 (goat).

Could you please let me know what kind of IHC you are planning to pe...

Read More

1-10 of 11 Abreviews or Q&A

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